Professional Documents
Culture Documents
OBJECTIVES
1. 2. 3. To study the demand of MD2 pineapple locally and internationally. To show how micropropagation can overcome shortage in planting material for large-scale production of MD2 pineapple. To determine of any the steps in micropropagation can be automated or mechanized to overcome the high cost of labour and meet the high demand of the planting materials.
Introduction
Pineapple= Anana comosus Pineapple is one of the most important tropical fruit crops in the world. It has excellent nutritive qualities and good taste and these characteristics result in a fruit that is in great demand in the international market. in Malaysia ,cultivars like Sarawak (Smooth Cayenne), Josapine, N36, Moris and Gandol are commercially planted through conventional vegetative propagation using the crown and suckers .
MD2 PINEAPPLE
MD2 is a hybrid developed by the Hawaiian Pineapple Research Institute. MD stands for Mrs. Dillard- the wife of its producer. resistant to internal browning, which is the general problem faced in the pineapple production(Duval et al., 2001).
MD2 Pineapple
MD2, also named Golden Ripe. can be propagated using micropropagation, which is a type of tissue culture technology to regenerate millions of pineapple plantlets from cuttings or slips rather than propagating from seeds.
MD2 Pineapple
Benefits
color and flavor superior to other varieties. gives a medium to large (1.3-2.5 kg) cylindrical, square-shouldered fruit, with large flat eyes, and an intense orangeyellow color. The clear yellow pulp is sweet, compact, and fibrous. high in sugar and ascorbic acid but lower in total acid than Smooth Cayenne. resistant to internal browning,
The Demand
MD2 pineapple is the most highly demand cultivar after Smooth Cayenne due to its benefits. Beginning in 2002, Ghana farmers set out to rapidly expand production of MD2 because market demand for Smooth Cayenne fruit from Ghana had dropped sharply after MD2 won the major market share in Europe.
The Demand
The Costa Rican MD2 or Extra Sweet variety is now in a dominant position on the market. Traditionally producers of Smooth Cayenne African countries are now turning to new varieties to meet European demand and to secure a foothold on the MD2 (Jacob & Soman, 2006).
The Demand
NCER has embarked on MD2 pineapple cultivation where the farm was harvested about 22,500 Metric tonnes in July 2011. They are interested in promoting the products to potential importers and had provided 5,000 pieces of MD2 sample for the United Kingdom market test and approval.
Cont
the importation of suckers for direct planting by farmers are expensive The cost of a plantlet is dependent on the cultivar and its size For MD2 cultivars, the propagules are expensive as it is the best cultivar among pineapple varieties.
Propagation
Conventional Micropropagation
CONVENTIONAL
Crown (quartering) Slips Suckers Ratoons
MICROPAGATION
ORGANOGENESIS EMBRYOGENESIS Somatic embryogenesis is a regeneration process of somatic cells that are developed by cell division to form complete embryos which are analogous to zygotic embryos. Direct / indirect Emryo like structures develop into whole plant.
Organogenesis is a process to form and to develop shoots from meristematic tissue (Pardal, 2002). Relies on the production of organs, directly or from explants.
Advantages
micropropagated plants are more or less genetically homogeneous, shoot tip culture allows the plants to become virus free. the plants can be produced irrespective of weather, season and other environment conditions. allows higher efficiency of production, in a short time span (Ammirato, 1983).
Disadvantages
high cost production and have limited the use and exploitation of this technique at industrial level. Hence, the most challenging aspect at present is to reduce the production cost, thereby improving the production efficiency (Ziv, 1989). Expert needed.
(TIS)
The main advantage is the reduction of the hyperhydricity. Immersion time, such as duration or frequency is the most critical parameter for system efficiency. improves plant tissue quality Temporary immersion system combined the advantages of solid and liquid medium culture. Allows explant aeration but limited contact with nutrients & permits an efficient nutrient uptake.
METHODOLOGY
1. Information sources 2. Questionaire distribution 3. Data analyzing
100000
80000
20000
0 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010
Statistic of Exportation of Malaysian Pineapple to Singapore and West Asia (Metric Tonnes)
80000 70000
60000
50000
40000
30000
20000
10000
0 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010
US imports of Fresh and Frozen and Canned Pineapples, 1997-2007 (in pounds and $ US)
Micropropagation as the Solution to Meet the High Demand of MD2 Planting Materials
It is reported that starting with 30 explants it is possible to produced 1,250,000 pineapple plantlets within eight months of in vitro multiplication. The micropropagation protocol has now been standardized for most of the important pineapple.
Acclimatization and Nursing operation Automated soil mixture Automated planting container system automated misting system
RITA temporary immersion system (TIS) was integrated to reduce the labour & increase the efficiency. MD2 cultivation for both local and international should be encouraged because MD2 variety has a great potential in the horticultural industry